Chemoenzymatic design of heparan sulfate oligosaccharides.

نویسندگان

Renpeng Liu

Yongmei Xu

Miao Chen

Michel Weïwer

Xianxuan Zhou

Arlene S Bridges

Paul L DeAngelis

Qisheng Zhang

Robert J Linhardt

Jian Liu

چکیده

Heparan sulfate is a sulfated glycan that exhibits essential physiological functions. Interrogation of the specificity of heparan sulfate-mediated activities demands a library of structurally defined oligosaccharides. Chemical synthesis of large heparan sulfate oligosaccharides remains challenging. We report the synthesis of oligosaccharides with different sulfation patterns and sizes from a disaccharide building block using glycosyltransferases, heparan sulfate C(5)-epimerase, and sulfotransferases. This method offers a generic approach to prepare heparan sulfate oligosaccharides possessing predictable structures.

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